Stalk Analyzer
Spalding Lab @ University of Wisconsin
Overview
| Measured variables | count, diameter |
|---|---|
| Operating system | mac, windows, matlab, linux |
| Licence | open-source, freeware |
| Automation level | semi-automated, automated |
| Plant requirements | monocot |
| Export formats | txt |
| Other information | - |
Scientific article(s)
Image analysis of anatomical traits in stalk transections of maize and other grassesSven Heckwolf, Marlies Heckwolf, Shawn M Kaeppler, Natalia de Leon, Edgar P SpaldingPlant Methods, 2015 View paper
Description
To meet the need for large-scale measurements of stalk anatomy features, we developed custom image processing software that utilized a variety of global thresholding, local filtering, and feature detection methods to measure rind thickness, pith area, vascular bundle counts, and individual vascular bundle size from digital images of hand-cut transections of stalks collected with a flatbed document scanner. The tool determined vascular bundle number with an average accuracy of 90% across maize genotypes that varied five-fold for this trait. The method is demonstrated on maize, sorghum, and Miscanthus stalks.
Source: Plan Methods