HyphaTracker
Overview
| Measured variables | growth |
|---|---|
| Operating system | windows, mac, linux |
| Licence | open-source, freeware |
| Automation level | automated |
| Plant requirements | - |
| Export formats | txt |
| Other information | Imagej macro |
Scientific article(s)
HyphaTracker: An ImageJ toolbox for time-resolved analysis of spore germination in filamentous fungiMichael Brunk,Sebastian Sputh,Sören Doose,Sebastian van de Linde,Ulrich TerpitzScientific Reports, 2018 View paper
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Description
HyphaTracker allows for processing of microscopic acquisitions (movies) of conidial germination starting with drift correction and data reduction prior to germling analysis. From the image time series germling related region of interests (ROIs) are extracted, which are analysed for their area, circularity, and timing. ROIs originating from germlings crossing other hyphae or the image boundaries are omitted during analysis. Each conidium/hypha is identified and related to its origin, thus allowing subsequent categorization.